Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis
P. Garcia-Reitboeck, A. Phillips, T.M. Piers, C. Villegas-Llerena, M. Butler, A. Mallach, C. Rodrigues, C.E. Arber, A. Heslegrave, H. Zetterberg, H. Neumann, S. Neame, H. Houlden, J. Hardy and J.M. Pocock
Garcia-Reitboeck et al. describe the generation of human induced pluripotent stem cell-derived microglia-like cells from patients with early-onset dementia caused by variants in the immune receptor gene TREM2. They observed functional deficits in TREM2 variant cells, including reduced soluble TREM2 secretion, selectively reduced phagocytosis of apoptotic neuronal cells, and a deficit in migratory capacity.